Immobilization of vaginal Lactobacillus in polymeric nanofibers for its incorporation in vaginal probiotic products

Abstract Probiotic products require high number of viable and active microorganisms during storage. In this work, the survival human vaginal Lactobacillus gasseri CRL1320 rhamnosus CRL1332 after nanofiber-immobilization by electrospinning with polyvinyl-alcohol, storage was evaluated. The optimization bacterial immobilization conditions using bioprotectors (skim milk-lactose glycerol) oxygen-excluding packaging carried out, compared lyophilization. After electrospinning, a higher rate L. (93%) to (84%) obtained in nanofibers, cells (>107 colony-forming unit/g) two probiotics nanofibers stored at -20°C up 14 days. an excellent strategy extend shelf-life (up 1.7 × 108 CFU/g) 4°C 360 days, no addition bioprotectives, resulting similar freeze-dried-cells. successfully incorporated into polymeric hydrophilic mean diameter 95 nm. composite materials were characterized terms morphology, their physicochemical thermal properties assessed. Nanofiber-immobilized maintained inhibition urogenital pathogens. Thus, nanofiber-immobilized can be included probiotic prevent or treat infections.